The NLD says Ms Suu Kyi, 63, has not been able to sleep at night because of continuing cramps in her legs and needs urgent medical attention.
Ms Suu Kyi is on trial for allegedly violating the terms of her house arrest after a US man broke into her home.
She denies the charges and faces five years in prison if found guilty.
Ms Suu Kyi has suffered from ill-health in the past. Shortly before her arrest on 14 May she was treated for dehydration and low blood pressure.
The NLD said she was "in desperate need of proper medical treatment".
"We are very much concerned about her health," it said in a statement.
Ms Suu Kyi has been under house arrest and banned from seeing all but a small group of people for 13 of the past 19 years.
She was arrested on 14 May after an American man swam across the lake to reach her compound, spending two nights there.
Critics of the trial say it is being used as an excuse to keep Ms Suu Kyi in detention until after elections scheduled for next year.
But Burma's ruling junta has rejected the international condemnation, saying the trial is "an internal legal issue".
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